fluorescence photo-oxidation, quantum dots, double labelling with both fluorescent and gold conjugated antibodies, or fluoronanogold. Ultrathin sections of tissue are treated with a label that is able to be imaged under both microscopes, e.g. This technique pairs the high resolution of the electron microscope which is able to reveal the cellular ultrastructure with the ability to follow specific targets on or in living cells, revealing dynamic localisation and/or function of target molecules using fluorescence microscopy. Correlative TEMīreast cancer cells by Sharon Lequeuex, read more below.Ĭorrelative microscopy involves using multiple microscope systems to observe the same specimen, most commonly light and transmission electron microscopy. The cytoplasm is full of mitochondria, lipid droplets, transparent vesicles, and an MII nucleus. This oocyte is surrounded by expanding cumulus cells and a zona pellucida layer. I have been studying the ultrastructural differences between adult and prepubertal lamb oocytes to find reasons for the reduced ability of juvenile oocytes to produce viable embryos. Image of maturing sheep oocyte by Karen Reader Depending on the sample, the CM100 TEM is able to resolve images down to a few nanometres. To prepare samples for TEM the specimens are typically fixed in glutaraldehyde, stained with uranyl acetate, embedded in resin and then cut into sections 70-80nm thin. TEM can also provide crystallographic information about the sample such as the arrangement of atoms in the specimen and their degree of order, including detection of atomic-scale defects in areas a few nanometres in diameter. From this we can learn about the morphology of the specimen such as the size, shape and arrangement of the particles which make up the sample as well as their relationship to each other. Areas of the specimen that are more electron dense allow fewer transmitted unscattered electrons and appear darker, conversely the thinner areas and those containing lighter elements, permit more transmission and appear lighter. The transmission of unscattered electrons is a function of the specimen thickness and elemental composition. Maturing sheep oocyte by Karen Reader, read more below.Ī beam of electrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through. Visit Scanning electron microscopy techniques for SEM techniques. This page describe the techniques our transmission electron microscopes can provide.
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